Nex-Gen Core: Next Generation Sequencing Core Facility
UTMB’s Next Generation Sequencing (NGS) Core provides high throughput sequencing to UTMB investigators and others using dedicated Element Biosciences’ Aviti sequencing instrument and Illumina NextSeq 2000, NextSeq 550 (this will be retired by the end of FY 24) and MiniSeq instrument. Aviti offers lower cost and higher Qscore, while Illumina platforms are still the mainsteam technologies on the market. The NGS Core offers support in library construction from various template sources; RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq), and DNA (genomic and amplicon-derived). Library quality and quantity is assessed using Agilent Bioanalyzer and qPCR. Multiple platforms offer choices of different technologies and cost structures. Libraries prepared for Illumina sequencing can be sequenced on both technological platforms. In addition to short read sequencing, the core can also offer long read sequencing support. Please contact core if you are interested in long read sequencing (RNA isoform sequencing, full length 16s rRNA sequencing, and de novo genome assembly).
Request Forms
ISC Instructions
Next Generation Sequencing
Costs: October 1,2024
| Platform | Run Type | Lane/Flowcell* | Estimated Reads | Cost |
| Aviti | | | | |
| | PE300 | Lane | 50M | $1,475 |
| | PE300 | Flowcell | 100M | $2,350 |
| | PE300 | Lane | 150M | $1,850 |
| | PE300 | Flowcell | 300M | $3,100 |
| | PE150 | Lane | 125M | $775 |
| | PE150 | Flowcell | 250M | $1,250 |
| | PE75 | Lane | 250M | $875 |
| | PE75 | Flowcell | 500M | $1,450 |
| | PE75 | Lane | 500M | $1,200 |
| | PE75 | Flowcell | 1B | $2,100 |
| | PE150 | Lane | 250M | $1,050 |
| | PE150 | Flowcell | 500M | $1,800 |
| | PE150 | Lane | 500M | $1,400 |
| | PE150 | Flowcell | 1B | $2,500 |
* Aviti flowcells have two lanes that can be used for different libraries in the same run. For per lane sequencing, there will be a slight wait time to have two libraries for the same run type (same read length, same index read length, unless you have two pools).
| NextSeq 2000 | | |
| SE 100, XLEAP | 100M | $1,225 |
| PE 150, XLEAP | 100M | $1,420 |
| PE 300, XLEAP | 100M | $2,025 |
| SE 100, XLEAP | 400M | $1,556 |
| PE 100, XLEAP | 400M | $2,150 |
| PE 150, XLEAP | 400M | $2,250 |
| PE 300, XLEAP | 300M | $3,520 |
| SE 50 | 1200M | $2,700 |
| SE 100, XLEAP | 1200M | $2,200 |
| PE 100, XLEAP | 1200M | $3,220 |
| PE 150, XLEAP | 1200M | $3,300 |
| SE 50, XLEAP | 1800M | $2,450 |
| SE 100, XLEAP | 1800M | $2,900 |
| PE 100, XLEAP | 1800M | $4,000 |
| PE 150, XLEAP | 1800M | $4,300 |
M, million; SE, single end; PE, paired end
NGS Data Analysis $85/hr
| Library Cost | |
| RNA-Seq Non-Stranded, no dT selection | $276.00 |
| RNA-Seq Non-Stranded & dT Selected | $282.00 |
| RNA-Seq Stranded & dT Selected | $304.00 |
| RNA-Seq RiboZero rRNA Depletion | $373.00 |
| ChIP-Seq | $269.00 |
| DNA-Seq (Covaris) | $274.00 |
| DNA-Seq Nextera Flex | $278.00 |
| microRNA (Small RNA) | $296.00 |
Staff:
Jill K. Thompson
Haiping Hao, PhD
Location: 2.210 Basic Science Bldg.
Contact: hahao@utmb.edu
Phone: (409) 772-6349
Please contact Dr. Hao to discuss your sequencing project.